- Regular 21 - 35 day cycles
- Presence of suggestive symptoms (mittelschmerz, pre-menstrual, or dysmenorrhea)
- Presence of biphasic basal body temperature
- Positive serum progesterone
- Endometrial histology (secretory as opposed to proliferative)
Though all of these factors should be taken into account to evaluate ovulatory status, the first two are responsible for 95% of the evaluation. However, the only 100% effective method of proving ovulation is pregnancy.